General Cell Based Assays
Eurofins Discoverx PathHunter Human GPR148 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR148 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR148 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
BioVendor, LLC Sircol; Dye & Dye Release Reagents (2 x 110 ml, 2 x 150 ml). CONTAINS: Sircol; Dye Reagent (2 x 110 ml) AND Sircol™ Dye Release Reagents (2 x 150 ml). Biocolor Item # S1500
Sircol; Dye & Dye Release Reagents (2 x 110 ml, 2 x 150 ml). CONTAINS: Sircol; Dye Reagent (2 x 110 ml) AND Sircol™ Dye Release Reagents (2 x 150 ml). Sircol™ Collagen Assay components. Biocolor Item # S1500
Eurofins Discoverx PathHunter eXpress CMKLR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0313E2CP0L)
The PathHunter CMKLR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of CMKLR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CMKLR1 and EA-tagged β-Arrestin-2. Activation of CMKLR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter™ Human Apelin (APJ Receptor) Gi cAMP Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human Apelin (APJ Receptor) Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure AGTRL1 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human PPYR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human PPYR1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure PPYR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical CelBased asy MyeloperoxId 25uL
A peptide MC1R antagonist (IC50 17 nM) selectively binds to MC1R over MC3R and MC5R (IC50s 3900 and 1000 nM respectively) as well as MC4R at 10 UM increases the latency to tail withdrawal in the tail-flick test in female but not male mice in a model of opioid-induced hyperalgesia at 7.5 UG/animal i.c.v. reduces protein levels of merlin in the cortex reduces the number of surviving neurons in the cortex and hippocampal CA2 region and increases the number of apoptotic cells in the cortex in rats 24 hours after injury in a model of controlled cortical impact-induced TBI at 1 nmol/animal i.c.v.
Cayman Chemical Calcium Assay Kit, 192 Well
Cayman's Calcium Assay provides a quick, reliable method for determining total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates. The assay utilizes an optimized variant of the well-established o-Cresolphthalein-calcium reaction in which a vivid purple complex that absorbs between 560 and 590 nm is formed in the presence of calcium.
Bioassay Systems EnzyChrom™ L-Lactate Assay Kit. For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.
For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.05 mM.
Eurofins Discoverx PathHunter Bioassay Detection Kit, 15 (200 dp, 96-well). (93-0933E)
The PathHunter Bioassay Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter Bioassay cells or cell lines. This kit contains lysis buffer and EFC substrate to allow for detection of EFC levels in the assayed cells. This kit contains all components required to assay 2 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0933e.
Abcam Lysyl Oxidase Activity Assay Kit (Fluorometric)
Lysyl Oxidase Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Meso Scale Discovery U-PLEX Human Nectin-4 ASY
The Meso Scale Discovery Human Nectin-4 Singleplex Assay Kit is a singleplex immunoassay that measures Nectin-4 levels in human serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity wide dynamic range and low sample volume requirements The assay requires 50 L of sample and can be read on Meso Scale Discovery SECTOR Imagers The assay has a typical lower limit of detection of 0 03 ng/mL and an upper limit of quantification of 2500 ng/mL This assay kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator and control
Eurofins Discoverx PathHunter Human F2RL3 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human F2RL3 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure F2RL3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx InCELL Hunter eXpress CLK1 Kinase Binding Assay, 2pl Kit. (96-0059E1CP0M)
The InCELL Hunter eXpress CLK1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of CLK1 via detection of CLK1 levels. This product uses EFC technology. The cell line included overexpresses CLK1 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical γ-Glutamyl Transferase Activity Assay Kit, 1 ea
Cayman’s GGT Activity Assay Kit provides a convenient method of detecting GGT activity in plasma, serum, and tissue lysates. In this assay, GGT catalyzes the transfer of the γ-glutamyl group from L-γ-glutamyl-p-nitroaniline (γGpNA) to glycylglycine resulting in the liberation of the colored product p-nitroaniline (pNA), which can be measured at 418 nm. Under circumstances in which the GGT activity rate is limiting, the rate of increase is directly proportional to the GGT activity in the sample.
Meso Scale Discovery RPLEX HUM IGF2R/CIM6PR ASY
The Meso Scale Discovery IGF-2R (CI-MPR) Kit is an electrochemiluminescent immunoassay designed for the quantitative measurement of Insulin-like Growth Factor 2 Receptor (IGF-2R) in human serum plasma and cell culture supernatants This assay employs an MSD GOLD SULFO-TAG label and has a typical lower limit of detection of 0 03 ng/mL The kit includes a 96-well plate coated with an IGF-2R capture antibody a detection antibody conjugated to an MSD SULFO-TAG calibrators positive and negative controls 10X Read Buffer 10X Wash Buffer and Blocker A This assay can be run on the MESO QuickPlex SQ 120 MESO SECTOR S 600 or MESO SECTOR S 6000 instruments